Bonjour aux nouveaux membres Pourriez-vous mettre un avatar, merci

Data pre-processing

crowek
Messages : 8
Enregistré le : mar. 12 juin 2018 10:40

Data pre-processing

Message non lu par crowek » mar. 10 juil. 2018 11:36

Hello,

How do I pre-process the CyTOF data before clustering? I have FCS files that were trimmed for alive, singlet, leukocytes. After that, do I normalize the data so that the samples have similar expression patterns? If so, what are the different methods? Also, I see from the Cytofkit paper that the values are first transformed. Why are they transformed and what are the pros and cons of each transformation?

Please help

I didn't find the right solution from the internet.

References:
http://cytoforum.stanford.edu/viewtopic.php?f=3&t=1026
Motion Infographic Animation Company

Thanks

Répondre

Réseau social

     

Qui est en ligne

Utilisateurs parcourant ce forum : Aucun utilisateur enregistré et 0 invité