How do I pre-process the CyTOF data before clustering? I have FCS files that were trimmed for alive, singlet, leukocytes. After that, do I normalize the data so that the samples have similar expression patterns? If so, what are the different methods? Also, I see from the Cytofkit paper that the values are first transformed. Why are they transformed and what are the pros and cons of each transformation?
I didn't find the right solution from the internet.
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