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Data pre-processing

Messages : 8
Enregistré le : mar. 12 juin 2018 10:40

Data pre-processing

Message non lu par crowek » mar. 10 juil. 2018 11:36


How do I pre-process the CyTOF data before clustering? I have FCS files that were trimmed for alive, singlet, leukocytes. After that, do I normalize the data so that the samples have similar expression patterns? If so, what are the different methods? Also, I see from the Cytofkit paper that the values are first transformed. Why are they transformed and what are the pros and cons of each transformation?

Please help

I didn't find the right solution from the internet.

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